Review



har sequences  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92

    Structured Review

    Addgene inc har sequences
    Har Sequences, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/har+sequences/pPD189+VP16-ZF37+(Plasmid+%23138759)/10__1523_slash_jneurosci__1943___17__2017-58-32-67
    Average 92 stars, based on 2 article reviews
    har sequences - by Bioz Stars, 2026-09
    92/100 stars

    Images

    Related Articles

    Knock-In:

    Article Title: Optogenetic Activation of the fruitless-Labeled Circuitry in Drosophila subobscura Males Induces Mating Motor Acts
    Article Snippet: .. 135 136 Construction of a knock-in vector and the generation of knock-in fru alleles 137 To construct a donor vector for generating the frusoDR allele, 698 bp (HAL) and 138 759 bp (HAR) sequences flanking the predicted cut site of gRNA were amplified by 139 PCR and incorporated, with an In-Fusion HD Cloning kit (Takara Bio, Shiga, Japan), 140 into the EcoRI and XhoI sites of pDsRed-attP (Addgene plasmid; 51019), respectively. ..

    Plasmid Preparation:

    Article Title: Optogenetic Activation of the fruitless-Labeled Circuitry in Drosophila subobscura Males Induces Mating Motor Acts
    Article Snippet: .. 135 136 Construction of a knock-in vector and the generation of knock-in fru alleles 137 To construct a donor vector for generating the frusoDR allele, 698 bp (HAL) and 138 759 bp (HAR) sequences flanking the predicted cut site of gRNA were amplified by 139 PCR and incorporated, with an In-Fusion HD Cloning kit (Takara Bio, Shiga, Japan), 140 into the EcoRI and XhoI sites of pDsRed-attP (Addgene plasmid; 51019), respectively. ..

    Construct:

    Article Title: Optogenetic Activation of the fruitless-Labeled Circuitry in Drosophila subobscura Males Induces Mating Motor Acts
    Article Snippet: .. 135 136 Construction of a knock-in vector and the generation of knock-in fru alleles 137 To construct a donor vector for generating the frusoDR allele, 698 bp (HAL) and 138 759 bp (HAR) sequences flanking the predicted cut site of gRNA were amplified by 139 PCR and incorporated, with an In-Fusion HD Cloning kit (Takara Bio, Shiga, Japan), 140 into the EcoRI and XhoI sites of pDsRed-attP (Addgene plasmid; 51019), respectively. ..

    Amplification:

    Article Title: Optogenetic Activation of the fruitless-Labeled Circuitry in Drosophila subobscura Males Induces Mating Motor Acts
    Article Snippet: .. 135 136 Construction of a knock-in vector and the generation of knock-in fru alleles 137 To construct a donor vector for generating the frusoDR allele, 698 bp (HAL) and 138 759 bp (HAR) sequences flanking the predicted cut site of gRNA were amplified by 139 PCR and incorporated, with an In-Fusion HD Cloning kit (Takara Bio, Shiga, Japan), 140 into the EcoRI and XhoI sites of pDsRed-attP (Addgene plasmid; 51019), respectively. ..

    Polymerase Chain Reaction:

    Article Title: Optogenetic Activation of the fruitless-Labeled Circuitry in Drosophila subobscura Males Induces Mating Motor Acts
    Article Snippet: .. 135 136 Construction of a knock-in vector and the generation of knock-in fru alleles 137 To construct a donor vector for generating the frusoDR allele, 698 bp (HAL) and 138 759 bp (HAR) sequences flanking the predicted cut site of gRNA were amplified by 139 PCR and incorporated, with an In-Fusion HD Cloning kit (Takara Bio, Shiga, Japan), 140 into the EcoRI and XhoI sites of pDsRed-attP (Addgene plasmid; 51019), respectively. ..

    Cloning:

    Article Title: Optogenetic Activation of the fruitless-Labeled Circuitry in Drosophila subobscura Males Induces Mating Motor Acts
    Article Snippet: .. 135 136 Construction of a knock-in vector and the generation of knock-in fru alleles 137 To construct a donor vector for generating the frusoDR allele, 698 bp (HAL) and 138 759 bp (HAR) sequences flanking the predicted cut site of gRNA were amplified by 139 PCR and incorporated, with an In-Fusion HD Cloning kit (Takara Bio, Shiga, Japan), 140 into the EcoRI and XhoI sites of pDsRed-attP (Addgene plasmid; 51019), respectively. ..



    Similar Products

    90
    CustomArray Inc molecular inversion probes (mips) targeting flanking regions of all har sequences
    KEY RESOURCES TABLE
    Molecular Inversion Probes (Mips) Targeting Flanking Regions Of All Har Sequences, supplied by CustomArray Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/har+sequences/molecular+inversion+probes++mips++targeting+flanking+regions+of+all+har+sequences/pmc08542612-551-15-35
    Average 90 stars, based on 1 article reviews
    molecular inversion probes (mips) targeting flanking regions of all har sequences - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Promega har-smbit sequence
    KEY RESOURCES TABLE
    Har Smbit Sequence, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/har+sequences/har+smbit+sequence/pmc07865915-42-3-9
    Average 90 stars, based on 1 article reviews
    har-smbit sequence - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    92
    Addgene inc har sequences
    KEY RESOURCES TABLE
    Har Sequences, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/har+sequences/pPD189+VP16-ZF37+(Plasmid+%23138759)/10__1523_slash_jneurosci__1943___17__2017-58-32-67
    Average 92 stars, based on 1 article reviews
    har sequences - by Bioz Stars, 2026-09
    92/100 stars
      Buy from Supplier

    Image Search Results


    KEY RESOURCES TABLE

    Journal: Neuron

    Article Title: Rewiring of human neurodevelopmental gene regulatory programs by Human Accelerated Regions (HARs)

    doi: 10.1016/j.neuron.2021.08.005

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: CaptureMPRA library design, cloning, and transfection Molecular Inversion Probes (MIPs) targeting flanking regions of all HAR sequences were designed using the MIPgen program ( Boyle et al., 2014 ) and manufactured as a pool by CustomArray (Bothell, WA).

    Techniques: Virus, Recombinant, Electron Microscopy, Modification, Lysis, Sonication, Plasmid Preparation, Software

    KEY RESOURCES TABLE

    Journal: Neuron

    Article Title: Rewiring of human neurodevelopmental gene regulatory programs by Human Accelerated Regions (HARs)

    doi: 10.1016/j.neuron.2021.08.005

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: CaptureMPRA library design, cloning, and transfection Molecular Inversion Probes (MIPs) targeting flanking regions of all HAR sequences were designed using the MIPgen program ( Boyle et al., 2014 ) and manufactured as a pool by CustomArray (Bothell, WA).

    Techniques: Virus, Recombinant, Electron Microscopy, Modification, Lysis, Sonication, Plasmid Preparation, Software